• How to Measure Airborne Microbial Contamination Using Passive Sampling or the Settling Plate Technique

    A Comprehensive Literature Review & a Helpful Infographic

  • How To Use DIY Mould Test Kits

    It's easy, just follow the steps

    Infographic flowchart showing how to use the DIY mould testing kits

    Of course, if you have any questions, just call or email for advice

    You MUST use the kit within one (1) week of arrival. Petri plate nutrient media is like a foodstuff and will go off over time. In the event you do not use the kit promptly, you may need to re-order a new kit.

  • Literature Review of the Method

    Learn about how other people have used this method


    • Ejechi, B.O. and Ochei, O.P. (2017). Bacteriological safety assessment, hygienic habits and cross-contamination risks in a Nigerian urban sample of household kitchen environment. Environ Monit Assess. 189: 298. 
    • Vijayakumar, R., Saleh Al-Aboody, M., Alturaiki, W., Alsagaby, S.A. and Sandle, T. (2017). A study of airborne fungal allergens in sandstorm dust in Al-Zulfi, central region of Saudi Arabia. Journal of Environmental and Occupational Science. 6(1): 27-33. 
    • Wang, Y-H., Wang, Y-C., Wu, P-H., Hsu, L., Wang, C-Y., Jan, C-R. and Su, H-H. (2017). A cross-sectional study into the correlation of common household risk factors and allergic rhinitis in Taiwan's tropical environment. Asian Pacific Journal of Tropical Medicine. 10(2): 134-140. 
    • Molina-Veloso, A. and Borrego-Alonso, S.F. (2017). Viable allergenic fungi in a documentary deposit of the National Archive of Cuba. Revista Alergia Mexico. 64(1): 40-51.
    • Norbäck, D., Hisham Hashim, J.,  Cai, G-H., Hashim, Z., Ali, F., Bloom,E. and Larsson, L. (2016). Rhinitis, ocular, throat and dermal symptoms, headache and tiredness among students in schools from Johor Bahru, Malaysia: Associations with fungal DNA and mycotoxins in household dust. PLOS ONE. 1(11): 1-15
    • Luongo, J.C, Barberán, A, Hacker-Cary, R, Morgan, E.E., Miller, S.L. and Fierer, N. (2016). Microbial analyses of airborne dust collected from dormitory rooms predict the sex of occupants. Indoor Air. 28 March. Early View: 16th April 
    • Agodi, A. Auxilla, F., Barchitta, M., Cristina, M.L., D’Alessandro. D., Mura, I., Nobile, M., Pasquarella, C., Italian Study Group of Hospital Hygiene. (2015). Operating theatre ventilation systems and microbial air contamination in total joint replacement surgery: results of the GISIO-ISChIA study. J. Hospit Infect. 90(3): 213-219.
    • Fekadu, S. and Getachewu, B. (2015). Microbiological assessment of indoor air of teaching hospital wards: a case of Jimma University Specialised Hospital. Ethiopian Journal of Health Sciences. 25(2): 117-122.

    • Setlhare, G., Malebo, N., Shale, K. and Lues, R. (2014). Identification of airborne microbiota in selected areas in a health-care setting in South Africa. BMC Microbiology. 14: 100.

    • Napoli, C., Marcotrigiano, V. and Montagna, M.T. (2012). Air sampling procedures to evaluate microbial contamination: a comparison between active and passive methods in operating theatres. BMC Public Health. 12: 594.

    • Napoli, C. (2012). Prevention of healthcare-associated infections: which sampling method should be used to evaluate air bio-contamination in operating rooms? Epidemiology. 2:2.
    • Burr, H.N., Wolf, F.R. and Lipman, N.S. (2012). Corynebacterium bovis: Epizootiologic features and environmental contamination in an enzootically infected rodent room. Journal of the American Association for Laboratory Animal Science. 51(2): 189-198.

    • Brandys, R. C. and Brandys, G.M. (2011). Worldwide exposure standards for mold and bacteria - with assessment guidelines for air, water, dust, ductwork, carpet, insulation and bulk materials. 9th ed. Occupational and Environmental Health Consulting Services Inc, OEHCS


    • Bowling, F.L., Stickings, D.S., Edwards-Jones, V., Armstrong, D.G. and Boulton, A.J.M. (2009). Hydrodebridement of wounds: effectiveness in reducing would bacterial contamination and potential for air bacterial contamination. Journal of Foot and Ankle Research. 2: 13.

    • Khojasteh, V.J., Edwards-Jones, V., Childs, C. and Foster, H.A. (2007). Prevalence of toxin producing strains of Staphylococcus aureus in a pediatric burns unit. Burns. 33(3): 334-340. 

    • Andon, B.M. (2006). Active air vs. passive air (settle plate) monitoring in routine environmental monitoring programs. PDA J Pharm Sci Technol. 60(6): 350-355. 

    • Sandle, T. (2006). Environmental monitoring risk assessment. Journal of GXP Compliance. 10(2): 54-73.


    • ISO 14698-1:2003. Cleanrooms and associated controlled environments - biocontamination control - part 1: general principles and methods. [Note that this Standard remains current in 2015] and specifies the use of a "Suitable container (e.g. Petri dish) of appropriate size, containing an appropriate, sterile, culture medium which is left open for a defined period to collect viable particles depositing from the air. ISO 14698-1".
    • Pasquarella, C., Masia, M.D., Nnanga, N., Sansebastiano, G.E., Savino, A., Signorelli, C., Veronesi, L. (2004). Microbial air monitoring in operating theatre: active and passive samplings. Ann Ig. 16(1-2): 375-386. 

    • Pasquarella, C., Pitzurra, O. and Savino, A. (2000). The index of microbial air contamination. Journal of Hospital Infection. 46: 241-256. 

All Posts
Our Service Terms & Conditions (T&C) are as follows for mould.net.au and these share all of the key T&C’s as used by our parent Company, Biological Health Services (BHS).  A summary of the key T&C’s used by mould.net.au include all of the following but are not limited to:

1. mould.net.au has a policy and procedure for promoting scientific integrity. Scientific integrity refers to maintaining the quality and objectivity of the inspection and analytical tasks involved with indoor air quality and associated microbiological or other investigations. It is important that such results are written up so they are ethical, sound and worthy of the public’s confidence. All reports are therefore premised on a commitment to sound, objective science where findings are objective, credible, unbiased and transparent. Requests to fabricate, falsify or omit information or alter reports or data will not be tolerated and will be immediate grounds for contract termination on the grounds of client misconduct. We will also not abridge findings, conclusions or recommendations into "short summaries" for clients and the formatting of reports is designed to faithfully detail all quantitative data and results and show how they were interpreted.

2. The client agrees to indemnify mould.net.au and keep mould.net.au indemnified against any loss, damage (including special or consequential loss or damage), costs (including legal fees), expenses, demands or liability for any claim by a third party or parties as a result of or arising from the client's use of the BHS report/interim findings/opinions or recommendations/implementation or scope of works other than in accordance with the terms of this agreement.

3. Nothing in this agreement constitutes either party (mould.net.au or client) a partner agent, employee or representative of the other party or creates any trust, association or partnership at law or for any purpose whatsoever nor do the parties intend that their activities under this service terms agreement or other social linkage constitute a business, financial operation or venture.

4. Copyright to written reports remains with the author. Full payment to written material and reports is required before release to client for practical use.

5. At mould.net.au the security of personal information that we collect is of utmost importance to us and we follow the Australian Privacy Principles and comply with the Privacy Act 1988 (Cth). In line with our Privacy Policy, mould.net.au may hold your personal information in electronic or hard copy format or a combination of both. We have taken a number of physical, electronic and procedural steps to protect your personal information from misuse, interference, loss, unauthorised access, modification or disclosure. mould.net.au will not disclose your credit card details to any other persons.

6. Reports are generally delivered approx. 3-4 business weeks after inspection and data/sample collection or sample delivery to our office and the time-frame for report delivery depends on volume of current work and other factors such as the urgency of results and their implications. Your patience is appreciated.

7. In the event that any written report we have already supplied to the client requires formatting modifications for other audiences, then such modifications and additions are charged at an hourly rate (example: Expert Evidence submissions).

8. All samples (swabs, direct samples, air-o-cell, tape-lifts, or any other matter for analysis) is only accepted on delivery at our Head Office (Level 1, 459 Toorak Rd, Toorak, VIC, 3142) address within normal business hours (8:30am-5:30pm Mon-Fri). No samples will be accepted at our lab address, which is a secure P1/2 facility for staff only. A chain of custody form must be completed for all samples accepted at our Head Office. Chain of custody forms will, be supplied electronically on request. All samples must be lawfully obtained.

9. Mail order lab tests will be shipped via Australia Post as quickly as practicable after order receipt.  There may be a delay of up to 5 working days in the event that stocks of fresh petri plate media are required.  Although we maintain ample reserves, from time to time, we may run low, and only send out petri plate media that is within its use-by date. Your patience is appreciated.  With the COVID-19 pandemic, we may have supply chain delays which are unavoidable and we apologize for any extra delays that may occur that are beyond our control.

10. Mail order lab tests (mould.net.au) must be promptly returned within a reasonable time frame expected for environmental microbiology samples that is normally 7-days by the client for analysis after receipt. If the mail-order testing services (perishable items/test media) are canceled after the testing kits have been shipped out or collected, please note that no refunds are provided.  

11. You MUST use the kit within one (1) week of arrival. Petri plate nutrient media is like a foodstuff and will go off over time. In the event you do not use the kit promptly, you may need to re-order a new kit. We do not supply new plates if you have not used the test supplies promptly after delivery.

12. This website or documents arising from our lab inspections or other consulting services for you, the client, may contain general information about medical conditions and treatments. This information is not advice, and should not be treated as such. It is provided “as is” without any representations or warranties, express or implied. You must not rely on information on this website or in documentation we provide to you as an alternative to medical advice from your doctor or other professional healthcare provider. If you have any specific questions about any medical matter you should consult your doctor or other professional healthcare provider. If you think you may be suffering from any medical condition you should seek immediate medical attention. You should never delay seeking medical advice, disregard medical advice, or discontinue medical treatment because of information on this website or arising from documentation or verbal statements we may provide to you.

13. Consumer rights principles are important to us, and in the event that you experience a problem, please contact us to discuss. Unresolved disputes will proceed to mediation as required for resolution.

14. All biological samples will be autoclaved after analysis or disposed of as biological waste or in some cases may be stored permanently as reference cultures or viable and/or non-viable cultures/samples having biotechnological or other intellectual property significance. The client acknowledges that all biological environmental samples from the time of sample acquisition become the property of Biological Health Services and that the client agrees to waive all current and future rights to ownership of any isolated cell lines or biological material components. These isolates are added to the Biobank and may be used for research or other commercial purposes.

15. Social engagement and education (also known as #edtech) is an important component of our customer service efforts. We reserve the right to take photographs, video and audio during any aspect of our work on any jobsite and engage with any persons we may interact with during the course of our work and you acknowledge that we may share these without notification to you on various different social media platforms.

16. This is not a full or complete list of our Service Terms & Conditions and the client acknowledges that any printed Service Terms on brochures, flyers, or similar may be an abridged version of this primary list and that you have understood, accepted and agreed to all such terms and conditions.  Our main website should be consulted for an up to date set of service terms and conditions.  
- Updated May, 2018

This following document sets forth the Privacy Policy for the Biological Health Services website, http://www.mould.net.au.

Biological Health Services is committed to providing you with the best possible customer service experience. Biological Health Services is bound by the Privacy Act 1988 (Cth), which sets out a number of principles concerning the privacy of individuals.

Collection of your personal information
There are many aspects of the site which can be viewed without providing personal information, however, for access to future Biological Health Services customer support features you are required to submit personally identifiable information. This may include but not limited to a unique username and password, or provide sensitive information in the recovery of your lost password.

Sharing of your personal information
We may occasionally hire other companies to provide services on our behalf, including but not limited to handling customer support enquiries, processing transactions or customer freight shipping. Those companies will be permitted to obtain only the personal information they need to deliver the service. Biological Health Services takes reasonable steps to ensure that these organisations are bound by confidentiality and privacy obligations in relation to the protection of your personal information.

Use of your personal information
For each visitor to reach the site, we expressively collect the following non-personally identifiable information, including but not limited to browser type, version and language, operating system, pages viewed while browsing the Site, page access times and referring website address. This collected information is used solely internally for the purpose of gauging visitor traffic, trends and delivering personalized content to you while you are at this Site.

From time to time, we may use customer information for new, unanticipated uses not previously disclosed in our privacy notice. If our information practices change at some time in the future we will use for these new purposes only, data collected from the time of the policy change forward will adhere to our updated practices.

Changes to this Privacy Policy
Biological Health Services reserves the right to make amendments to this Privacy Policy at any time. If you have objections to the Privacy Policy, you should not access or use the Site.

Accessing Your Personal Information
You have a right to access your personal information, subject to exceptions allowed by law. If you would like to do so, please let us know. You may be required to put your request in writing for security reasons. Biological Health Services reserves the right to charge a fee for searching for, and providing access to, your information on a per request basis.

Contacting us
Biological Health Services welcomes your comments regarding this Privacy Policy. If you have any questions about this Privacy Policy and would like further information, please contact us by any of the following means during business hours Monday to Friday.

Call: 1300132350

Post: Attn: Privacy Policy,
Biological Health Services,
Level 1, 459 Toorak Rd
Toorak, VIC, 3142

E-mail: [email protected]